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DNA probe Aac6를 이용한 A. actinomycetemcomitans ATCC 43177 및 ATCC 33277의 식별
Identification of A. actinomycetemcomitans ATCC 43177 and ATCC 33277 using DNA probe Aac6

구강생물학연구 2002년 26권 1호 p.221 ~ 230

임채광, 김미광, 최민호,

소속 상세정보

임채광 (  ) - 조선대학교 치과대학 법의치과학연구소
김미광 (  ) - 조선대학교 치과대학 구강생화학교실
최민호 (  ) - 조선대학교 치과대학 치과보철학교실

KMID : 0353320020260010221

Abstract

The purpose of this study was to isolate DNA probe which could identify Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) in Southern blot analysis. This study procedure includes (1) whole-genomic DNA extraction of A. actinomycetemcomitans ATCC 43717, A. actinomycetemcomitans ATCC 43718 and A. actinomycetemcomitans ATCC 33277 (2) construction of the genomic DNA library, (3) screening of restrictio fragment of genomid DNA, (4) identification of species-specific DNA probe by Southern blot hybridization. About 170 restriction fragments of several A. actinomycetemcomitans genomic DNA digested with the Hind III were obtained. One of them, the probe Aac6, restriction fragments of A. actinomycetemcomitans ATCC 33277, was shown to be hybridized with A. actinomycetemcomitans ATCC 43717, and A. actinomycetemcomitans ATCC 33277 but not A. actinomycetemcomitans ATCC 43728. From that result, the probe Aac6 may be useful in differentiation of A. actinomycetemcomitans ATCC 43717 and A. actinomycetemcomitans ATCC 33277 from A. actinomycetemcomitans ATCC 43178.